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Results and discussions

Covering the floor with polyethylene liner

The influence on intergranular gas composition, insect infestation and grain losses of covering the platform floor with a polyethylene liner is given in Table 1.

TABLE 1 - Influence of covering the platform floor with a polyethylene liner (duration of storage 8-10 months)

Gas concentration (%) 3-4 months after covering

Infestation1

Losses due to mould (%)

Total grain losses (%)

Liner

O2

CO2

Yes*

2-6

8-12

Rare

0.16 ± 0.1

0.32 ± 0.06

No**

6-9

2-4

Yes

0.13 ± 0.06

0.28 ± 0.06

* = data for 4 years; ** = data for 5 years.
1 Presence of adult insects in samples from the upper grain layers after covering with the PVC liner.

The data obtained over several years indicate that covering the floor with polyethylene significantly influences change in intergranular gas composition and also provides better control of insect infestations. It would not appear to have any serious influence on the amount of grain on the top of the platform damaged by mould or on total grain losses by the end of storage.

Duration of storage

The influence of the duration of storage on grain loss, infestation and grain germination is given in Table 2. This table shows that satisfactory insect control can be achieved for 1, 2 and 3 years of storage on a platform with a polyethylene covering over the floor. Grain germination remained very high (94-97%) even after 2 years of storage, and it was reduced to 88% only after 3 years of storage.

TABLE 2 - Influence of duration of storage on grain losses and other parameters (platform floor covered with a polyethylene liner)

Duration of grain storage (months)

Platform capacity (t)

Infestation1

Germination (%)

Losses (%)

Mould

Total

8-10*

2,500-4,000

Rare

95-97

0.16 ± 0.1

0.32 ± 0.06

23

2,500

Rare

94-98**

0.22

0.52

34

4 000

Rare

88 ± 1.6

0.66

0.96

* = data for 4 years; ** = viability.
1 Presence of adult insects in samples from the upper layers after opening the PVC liner.

TABLE 3 - Microflora and mycotoxins in barley samples of different degrees of deterioration at the end of a 10-month platform storage under a PVC liner

Parameter

Threshold value for the occurrence of symptoms of mycotoxicosis to animals

Sample A

Sample B

Sample C

Sample D

Taken from center of mould spot

Taken 10-20 cm below mould spot

Taken from grain surface free from any mould spot

Taken 0.5 m below grain surface

Visible signs of mould deterioration

Visible signs of mould deterioration

High moisture grain (17% m.c.)

Low moisture grain (12 % m.c.)

Very spoilt grain

Less spoilt grain

Sound grain

Sound grain

Mycotoxines (ppb)


T-2

200

0

0

0

0


Zearalenone

200

760

260

0

0


Deoxynivalenol

400

0

0

60

0


Fusarenon X

400

0

0

0

0


Nivalenol

200

0

0

350

0


Diacetoxyscirpenol

400

0

0

0

0


Ochratoxin A

100

1990

1570

0

0


Aflatoxin B1

20

0

0

0

0

Microflora (fungi number per g)

Total fungi


2.7 x 1010

5.7 x 109

2.2 x 106

1.6 x 106


Fusarium


5.5 x 104

1.5 x 104

4.5 x 105

5.5 x 105


Alternaria


9.2 x 107

4.2 x 107

1.2 x 104

0.7 x 104


Penicillium


2.4 x 1010

5.5 x 109

1.7 x 106

1.1 x 106


Aspergillus


3.2 x 108

9.7 x 107

9.7 x 104

4.7 x 104


F. graminearum


Few

Few

3.7 x 105

3.8 x 105


F. colmorum


Few

Few

0.7 x 105

1.7 x 105


F. moniliforme


Few

Few

9.5 x 104

2.5 x 104


P. ochraceus


2.4 x 1010

4.4 x 109

1.4 x 106

0.8 x 106


P. urticea


3.1 x 109

7.2 x 108

0.2 x 106

0.2 x 106


Mucor


Many

Many

Few

Few

On the other hand, the duration of storage had a significant influence on both mould losses and total loss. At the end of 3 years of storage, mould losses were four times greater than average mould losses after 1 year of storage. Similarly, total loss after 3 years of storage was three times greater than average loss after 1 year of storage.

Microflora and mycotoxins

The initial temperature of grain stored on platforms was between 30-37°C, whereas the lowest ambient air temperature during the winter season was 2-6°C. This resulted in the establishment of strong temperature gradients within the bulk which in turn caused convection currents, leading to transfer of moisture to the peak area of the bulk and therefore to mould deterioration of the grain in some spots at the apex of the bulk. These spots were 5-50 cm deep.

Two samples of very spoilt barley and spoilt barley taken from these mould spots at a depth of 0-20 cm and two samples of sound barley at 17% m.c. and 12% m.c., respectively, were analysed for mycotoxins and microflora. The results are given in Table 3. These results demonstrate that spoilt grain from the mould spots could be seriously contaminated by zearalenone (260-760 ppb) and ochratoxin (1,600-2,000 ppb). Spoilt grain was contaminated primarily by Alternaria, Penicillium, Aspergillus and Mucor fungi. The quantity of spoilt grain at these spots was very small, but the importance of carefully removing all spoilt grain from the bulk surface must be emphasised.

Analyses of samples "C" and "D" show that the grain stored on platforms actually remains free from mycotoxins and is suitable for animal consumption.

Total costs of platform erection and hermetic storage of 4,000 t grain

The fixed cost of building a concrete platform (75 m by 25 m), together with the costs of PVC and polyethylene and the current cost for storing 4,000 t of barley for 1, 2 and 3 years are all shown in Table 4. The total per-year cost for storing 1 t of barley for a period of 3 years is C£1.23.

Table 5 shows the losses that occurred in 1992 and 1995 during storage of local barley in the open. Grain was affected by rain, and a part of it was completely destroyed. In the years 1992 and 1995, 65,500 t of barley stored in the open were affected by rain and, based on the weighted average loss of 5.23%, some 3,425 t were completely destroyed. The economic loss was about C£387,100.

Table 6 shows the estimated monetary saving in losses to the 65,500 t of barley, which in 1992 and 1995 remained in the open unprotected against the rain, had they been placed in hermetic storage. The total fixed and running costs for 1 year's hermetic storage of 65,500 t of local barley on a concrete platform under PVC is estimated to be about C£152,000. The economic loss due to the lack of appropriate protection was about C£387,100. In addition, hermetic storage of grain on platforms under PVC successfully protects the grain against insect, bird and rodent attacks and also prevents its contamination by mycotoxins.

TABLE 4 - Costs of building a concrete platform and of hermetic storage of 4,000 t of barley under PVC liner

Costs

Total cost

Depreciation (years)

Costs per 1 year storage (C£)

Costs per 2 years storage (C£)

Costs per 3 years storage (C£)

Fixed costs


Building platform

56,250

30

1,875

3,750

5,625


PVC sheet for covering grain

10,990

7

1,570

3,140

4,710


Polyethylene liner for covering floor

522

1

522

522

522


Total fixed costs



2,967

7,412

10,857

Current costs


Electricity to inload 3000 t

75


75

75

75


Labour to inroad 3000 t and level grain surface

1,711


1,711

1,711

1,711


Labour to cover grain and weld PVC sheet

1,100


1,100

1,100

1,100


Labour to cover floor with polyethylene liner

40


40

40

40


Labour to remove PVC sheet

155


155

155

155


Labour to inload grain into trucks using a tractor

800


800

800

800


Total current costs



3,881

3,881

3,881

Total of fixed + current costs


Total costs per 4,000 t



7,848

11,293

14,738


Total costs per 4,000 t per year



7,848

5,647

4,913


Total costs per t per year



1.96

1.41

1.23

Platform 25 m wide, 75 m long, 7 m high; C£1 = US$2.1.

TABLE 5 - Barley loss due to rain during open storage after harvest (1992 and 1995)

Year

Total quantity harvested (thousand t)

Quantity affected by rain

Quantity completely spoilt by rain

Losses (completely spoilt grain), %

Losses (completely spoilt grain, C£113/t) (C£ x 1,000)

(thousand t)

(%)

(t)

of total quantity

of quantity affected by rain

1992

161.4

48.3

30.0

2,463

1.5

5.1

278

1995

122.6

17.2

14.0

1,011

0.8

5.9

114

Average of 1992 and 1995

142

32.8

25.8*

1,737

1.2

5.23*

196

* = weighted average.

TABLE 6 - Economic effectiveness of grain storage using the hermetic method on platforms under PVC liners

1-year hermetic storage of 65,500 t of barley under PVC

C£ x 1,000 (C£ 1 = US$2.1)

Total cost (fixed + current): 65,500 t x C£1.96/t/year

128

Total losses alter 1-year hermetic storage: 65,500 t x 0.32% losses x C£ 113/t

24

Total (cost + losses)

152

Total losses due to deterioration by rain occurring in 1992 and 1995 during storage of 65,500 t of barley in the open 65,500 t x 5.23% losses x C£113/t

387


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